Authors: Qiaz Q. H. Hua, Dietmar Kültz, Kathryn Wiltshire, Zoe A. Doubleday, and Bronwyn M. Gillanders
Journal: Global Change Biology
Global warming is one of the most significant and widespread effects of climate change. While early life stages are particularly vulnerable to increasing temperatures, little is known about the molecular processes that underpin their capacity to adapt to temperature change during early development. Using a quantitative proteomics approach, we investigated the effects of thermal stress on octopus embryos. We exposed Octopus berrima embryos to different temperature treatments (control 19°C, current summer temperature 22°C, or future projected summer temperature 25°C) until hatching. By comparing their protein expression levels, we found that future projected temperatures significantly reduced levels of key eye proteins such as S-crystallin and retinol dehydrogenase 12, suggesting the embryonic octopuses had impaired vision at elevated temperature. We also found that this was coupled with a cellular stress response that included a significant elevation of proteins involved in molecular chaperoning and redox regulation. Energy resources were also redirected away from non-essential processes such as growth and digestion. These findings, taken together with the high embryonic mortality observed under the highest temperature, identify critical physiological functions of embryonic octopuses that may be impaired under future warming conditions. Our findings demonstrate the severity of the thermal impacts on the early life stages of octopuses as demonstrated by quantitative proteome changes that affect vision, protein chaperoning, redox regulation and energy metabolism as critical physiological functions that underlie the responses to thermal stress.
全球变暖是气候变化最显著、最广泛的影响之一。虽然早期生命阶段特别容易受到温度升高的影响，但人们对其在早期发育过程中适应温度变化的分子过程知之甚少。本文采用定量蛋白质组学方法研究了热应激对章鱼胚胎的影响。本文将章鱼胚胎置于不同的温度处理下（对照温度19°C，当前夏季温度22°C，或未来预估夏季温度25°C）直至孵化。通过比较它们的蛋白质表达水平，本文发现未来预估的温度显著降低了关键的眼部蛋白质水平，如S-晶状体蛋白和视黄醇脱氢酶12，这表明章鱼胚胎在温度升高时视觉受损。本文同时发现，这与细胞应激反应相结合，包括参与分子伴侣作用和氧化还原调节的蛋白质的显著升高。能源资源也被从生长和消化等非必要过程中转移出去。上述发现以及在最高温度下观察到的高胚胎死亡率，确定了章鱼胚胎的关键生理功能可能在未来气候变暖条件下受损。本文研究结果表明，热冲击对章鱼早期生命阶段的严重影响，正如蛋白质组的定量变化所证明的，这些影响视觉、蛋白质伴侣、氧化还原调节和能量代谢的关键生理功能，是章鱼对热应激反应的基础。
（实习生许若橦编译）
